Brazilian Journal of Pulmonology

ISSN (on-line): 1806-3756 | ISSN (printed): 1806-3713

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Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation?

O azul de metileno atenua a inflamação em pulmões não isquêmicos após transplante pulmonar?

Marcus da Matta Abreu1,a, Francine Maria de Almeida1,b, Kelli Borges dos Santos2,c, Emílio Augusto Campos Pereira de Assis3,d, Rafael Kenji Fonseca Hamada4,e, Fabio Biscegli Jatene1,f, Paulo Manuel Pêgo-Fernandes1,g, Rogerio Pazetti1,h

J Bras Pneumol.2018;44(5):378-382

Abstract PDF PT PDF EN Portuguese Text

Objective: To evaluate whether methylene blue (MB) could minimize the effects of ischemia-reperfusion injury in the nonischemic lung on a lung transplantation rodent model. Methods: Forty female Sprague-Dawley rats were divided into 20 donors and 20 recipients. The 20 recipient rats were divided into two groups (n = 10) according to the treatment (0.9% saline vs. 1% MB solutions). All animals underwent unilateral lung transplantation. Recipients received 2 mL of saline or MB intraperitoneally prior to transplantation. After 2 h of reperfusion, the animals were euthanized and histopathological and immunohistochemical analyses were performed in the nonischemic lung. Results: There was a significant decrease in inflammation-neutrophil count and intercellular adhesion molecule-1 (ICAM-1) expression in lung parenchyma were higher in the saline group in comparison with the MB group-and in apoptosis-caspase-3 expression was higher in the saline group and Bcl-2 expression was higher in MB group. Conclusions: MB is an effective drug for the protection of nonischemic lungs against inflammation and apoptosis following unilateral lung transplantation in rats.

 


Keywords: Reperfusion injury; Methylene blue; Lung transplantation; Apoptosis; Inflammation.

 


 

 


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